E03A0449 Mouse Anti Histone Antibody ELISA kit
Mouse Anti Histone Antibody ELISA kit is suitable for detecting samples from mouse species. Anti Histone Antibody can also be called HIS-Ab, AHA.
Specifications of Mouse Anti Histone Antibody ELISA kit
Product Information | |
Cat. No. | E03A0449 |
Product Name | Mouse Anti Histone Antibody ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 50 ng/mL | 1 vial |
STANDARD C (0.5mL) | 100 ng/mL | 1 vial |
STANDARD D (0.5mL) | 250 ng/mL | 1 vial |
STANDARD E (0.5mL) | 500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
AHA ELISA kit applies the competitive enzyme immunoassay technique utilizing a Histone antigen and an AHA-HRP conjugate. The assay sample and buffer are incubated together with AHA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AHA concentration since AHA from samples and AHA-HRP conjugate compete for the Histone antigen binding site. Since the number of sites is limited, as more sites are occupied by AHA from the sample, fewer sites are left to bind AHA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AHA concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Anti Histone Antibody ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 98-104% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-103 | |
1:4 | 95-107 | |
1:8 | 91-102 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between AHA and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Anti Histone Antibody ELISA kit
Citations of Mouse Anti Histone Antibody ELISA kit
E03A0449 has been referenced in the below publications:
Transplanted Human Umbilical Cord Mesenchymal Stem Cells Facilitate Lesion Repair in B6.Fas Mice.
Relationship between p16 protein expression and prognosis in patients with gastric adenocarcinoma treated with XELOX chemotherapy.
