E03A0433 Mouse Anti-Mullerian Hormone ELISA kit
The Mouse Anti-Mullerian Hormone ELISA kit can be used to identify samples from the mouse species. Anti-Mullerian Hormone can also be called AMH, MIS, antim, MIF, and anti-Mullerian hormone.
Specifications of Mouse Anti-Mullerian Hormone ELISA kit
Product Information | |
Cat. No. | E03A0433 |
Product Name | Mouse Anti-Mullerian Hormone ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25ng/ml |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/ml | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/ml | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/ml | 1 vial |
STANDARD E (0.5mL) | 10 ng/ml | 1 vial |
STANDARD F (0.5mL) | 25 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
AMH ELISA kit uses an anti-AMH antibody and an AMH-HRP conjugate in a competitive enzyme immunoassay method. AMH-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-AMH antibody binding site between AMH from samples and AMH-HRP conjugate, the intensity of the color is inversely proportional to the concentration of AMH. Since the number of sites is limited, as more sites are occupied by AMH from the sample, fewer sites are left to bind AMH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMH concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Anti-Mullerian Hormone ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between AMH and analogues was observed. | |
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Summary of the Assay Procedure for Mouse Anti-Mullerian Hormone ELISA kit
Citations of Mouse Anti-Mullerian Hormone ELISA kit
E03A0433 has been referenced in the below publications：
The effect of the murine ovarian vitrification allograft for ovarian function in ovarian failure rat model.
