E03A0436 Mouse Anti Smooth Muscle Antibody ELISA kit
Mouse Anti Smooth Muscle Antibody ELISA kit is suitable for detecting samples from mouse species. Anti Smooth Muscle Antibody can also be called alpha sarcomeric Actin, alpha smooth muscle Actin, Actin alpha, ASMA, ASM-A, alpha-SMA, alpha SMA, AAT6, ACTA2, Actin alpha 2 smooth muscle aorta, Actin aortic smooth muscle, ACTSA, ACTVS, Alpha 2 actin.
Product Information | |
Cat. No. | E03A0436 |
Product Name | Mouse Anti Smooth Muscle Antibody ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 5-100ng/ml |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ASMA ELISA kit applies the competitive enzyme immunoassay technique utilizing a Smooth Muscle antigen and an ASMA-HRP conjugate. The assay sample and buffer are incubated together with ASMA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ASMA concentration since ASMA from samples and ASMA-HRP conjugate compete for the Smooth Muscle antigen binding site. Since the number of sites is limited, as more sites are occupied by ASMA from the sample, fewer sites are left to bind ASMA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ASMA concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 98-104% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-103 | |
1:4 | 95-107 | |
1:8 | 91-102 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ASMA and analogues was observed. | |
E03A0436 has been referenced in the below publications:
Medullary thymic epithelial cell depletion leads to autoimmune hepatitis.
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