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  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Anti Smooth Muscle Antibody ELISA kit (E03A0436)

E03A0436 Mouse Anti Smooth Muscle Antibody ELISA kit

Mouse Anti Smooth Muscle Antibody ELISA kit is suitable for detecting samples from mouse species. Anti Smooth Muscle Antibody can also be called alpha sarcomeric Actin, alpha smooth muscle Actin, Actin alpha, ASMA, ASM-A, alpha-SMA, alpha SMA, AAT6, ACTA2, Actin alpha 2 smooth muscle aorta, Actin aortic smooth muscle, ACTSA, ACTVS, Alpha 2 actin.

Products

Specifications of Mouse Anti Smooth Muscle Antibody ELISA kit

Product Information

Cat. No.

E03A0436

Product Name

Mouse Anti Smooth Muscle Antibody ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

5-100ng/ml

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ASMA ELISA kit applies the competitive enzyme immunoassay technique utilizing a Smooth Muscle antigen and an ASMA-HRP conjugate. The assay sample and buffer are incubated together with ASMA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ASMA concentration since ASMA from samples and ASMA-HRP conjugate compete for the Smooth Muscle antigen binding site. Since the number of sites is limited, as more sites are occupied by ASMA from the sample, fewer sites are left to bind ASMA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ASMA concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Anti Smooth Muscle Antibody ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

98-104%

Linearity

Diluent Ratio

Range %

1:2

96-103

1:4

95-107

1:8

91-102

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ASMA and analogues was observed.


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Summary of the Assay Procedure for Mouse Anti Smooth Muscle Antibody ELISA kit

Summary of the Assay Procedure for Mouse Anti Smooth Muscle Antibody ELISA kit

Citations of Mouse Anti Smooth Muscle Antibody ELISA kit

E03A0436 has been referenced in the below publications:

Medullary thymic epithelial cell depletion leads to autoimmune hepatitis.

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