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BlueGene Biotech Mouse Apolipoprotein A1 ELISA kit (E03A0509)

E03A0509 Mouse Apolipoprotein A1 ELISA kit

The Mouse Apolipoprotein A1 ELISA kit can be used to identify samples from the mouse species. Apolipoprotein A1 can also be called  APOA1, entrez:335, apo(a), apolipoprotein A1, Apolipoprotein A-I, HPALP2.

Products

Specifications of Mouse Apolipoprotein A1 ELISA kit

Product Information

Cat. No.

E03A0509

Product Name

Mouse Apolipoprotein A1 ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

5-100ug/ml

Sensitivity

1.0ug/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ug/mL

1 vial

STANDARD B (0.5mL)

5.0 ug/mL

1 vial

STANDARD C (0.5mL)

10 ug/mL

1 vial

STANDARD D (0.5mL)

25 ug/mL

1 vial

STANDARD E (0.5mL)

50 ug/mL

1 vial

STANDARD F (0.5mL)

100 ug/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

APOA1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-APOA1 antibody and an APOA1-HRP conjugate. The assay sample and buffer are incubated together with APOA1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APOA1 concentration since APOA1 from samples and APOA1-HRP conjugate compete for the anti-APOA1 antibody binding site. Since the number of sites is limited, as more sites are occupied by APOA1 from the sample, fewer sites are left to bind APOA1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APOA1 concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Apolipoprotein A1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between APOA1 and analogues was observed.


BlueGene Biotech Product Show

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Citations of Mouse Apolipoprotein A1 ELISA kit

E03A0509 has been referenced in the below publications:

Eff ects of Medium- chain Fatty Acid on Lipoproteins in C57BL6J Mice Fed High Fat Diet for Short andLong Duration.

Triacetyl-3-hydroxyphenyladenosine, a derivative of cordycepin,attenuates atherosclerosis in apolipoprotein E-knockout mice.

Simvastatin reduces atherogenesis and promotes the expression of hepatic genes associated with reverse cholesterol transport in apoE-knockout mice fed high-fat diet.

Effects of medium-chain fatty acids on cholesterol metabolism and their mechanisms in mice.


 


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