E03A0511 Mouse Apolipoprotein A4 ELISA kit
The Mouse Apolipoprotein A4 ELISA kit can be used to identify samples from the mouse species. Apolipoprotein A4 can also be called Apo-AIV, ApoA-IV, apoAIV, APOA4 .
Specifications of Mouse Apolipoprotein A4 ELISA kit
Product Information | |
Cat. No. | E03A0511 |
Product Name | Mouse Apolipoprotein A4 ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25ng/ml |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/ml | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/ml | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/ml | 1 vial |
STANDARD E (0.5mL) | 10 ng/ml | 1 vial |
STANDARD F (0.5mL) | 25 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
APOA4 ELISA kit uses an anti-APOA4 antibody and an APOA4-HRP conjugate in a competitive enzyme immunoassay method. APOA4-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-APOA4 antibody binding site between APOA4 from samples and APOA4-HRP conjugate, the intensity of the color is inversely proportional to the concentration of APOA4. Since the number of sites is limited, as more sites are occupied by APOA4 from the sample, fewer sites are left to bind APOA4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APOA4 concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Apolipoprotein A4 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between APOA4 and analogues was observed. | |
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Summary of the Assay Procedure for Mouse Apolipoprotein A4 ELISA kit
Citations of Mouse Apolipoprotein A4 ELISA kit
E03A0511 has been referenced in the below publications：
Environmental noise stress disturbs commensal microbiota homeostasis and induces oxi-inflammmation and AD-like neuropathology through epithelial barrier disruption in the EOAD mouse model.
