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  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Apolipoprotein B ELISA kit (E03A0513)

E03A0513 Mouse Apolipoprotein B ELISA kit

The Mouse Apolipoprotein B ELISA kit can be used to identify samples from the mouse species. Apolipoprotein B can also be called APOB, Apo-B, FLDB, Apo B-100, Apo B-48.

Products

Specifications of Mouse Apolipoprotein B ELISA kit

Product Information

Cat. No.

E03A0513

Product Name

Mouse Apolipoprotein B ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

2.5-50ug/ml

Sensitivity

0.1ug/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ug/mL

1 vial

STANDARD B (0.5mL)

2.5 ug/mL

1 vial

STANDARD C (0.5mL)

5.0 ug/mL

1 vial

STANDARD D (0.5mL)

10 ug/mL

1 vial

STANDARD E (0.5mL)

25 ug/mL

1 vial

STANDARD F (0.5mL)

50 ug/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

APOB ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-APOB antibody and an APOB-HRP conjugate. The assay sample and buffer are incubated together with APOB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APOB concentration since APOB from samples and APOB-HRP conjugate compete for the anti-APOB antibody binding site. Since the number of sites is limited, as more sites are occupied by APOB from the sample, fewer sites are left to bind APOB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APOB concentration in each sample is interpolated from this standard curve.


Quality Control on Mouse Apolipoprotein B ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between APOB and analogues was observed.


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Summary of the Assay Procedure for Mouse Apolipoprotein B ELISA kit

Summary of the Assay Procedure for Mouse Apolipoprotein B ELISA kit

Citations of Mouse Apolipoprotein B ELISA kit

E03A0513 has been referenced in the below publications:

Eff ects of Medium- chain Fatty Acid on Lipoproteins in C57BL6J Mice Fed High Fat Diet for Short andLong Duration .

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