E03A0690 Mouse Arachidonic Acid ELISA kit
The Mouse Arachidonic Acid ELISA kit can be used to identify samples from the mouse species. Arachidonic Acid can also be called arachidonic acid, arachidonate, (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoic acid, AA.
Product Information | |
Cat. No. | E03A0690 |
Product Name | Mouse Arachidonic Acid ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500pg/ml |
Sensitivity | 1.0pg/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 100 pg/ml | 1 vial |
STANDARD C (0.5mL) | 250 pg/ml | 1 vial |
STANDARD D (0.5mL) | 500 pg/ml | 1 vial |
STANDARD E (0.5mL) | 1000 pg/ml | 1 vial |
STANDARD F (0.5mL) | 2500 pg/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
AA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AA. Standards or samples are then added to the microtiter plate wells and AA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AA are added to each well to “sandwich” the AA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AA concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between AA and analogues was observed. | |
E03A0690 has been referenced in the below publications：
RhoA/Rho Kinase Mediates Neuronal Death Through Regulating cPLA2 Activation.
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