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  • bovine serum albumin elisa 1

BlueGene Biotech Mouse BCL2 Associated X Protein ELISA kit (E03B0032)

E03B0032 Mouse BCL2 Associated X Protein ELISA kit

The Mouse BCL2 Associated X Protein ELISA kit can be used to identify samples from the mouse species. BCL2 Associated X Protein can also be called BAX, BCL2L4, Bax Zeta, Apoptosis regulator BAX, Bcl-2-like protein 4.

Products

Specifications of Mouse BCL2 Associated X Protein ELISA kit

Product Information

Cat. No.

E03B0032

Product Name

Mouse BCL2 Associated X Protein ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

50-1000pg/ml

Sensitivity

1.0pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/ml1 vial

STANDARD B (0.5mL)

50 pg/ml

1 vial

STANDARD C (0.5mL)

100 pg/ml

1 vial

STANDARD D (0.5mL)

250 pg/ml

1 vial

STANDARD E (0.5mL)

500 pg/ml

1 vial

STANDARD F (0.5mL)

1000 pg/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

BAX ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-BAX antibody and an BAX-HRP conjugate. The assay sample and buffer are incubated together with BAX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BAX concentration since BAX from samples and BAX-HRP conjugate compete for the anti-BAX antibody binding site. Since the number of sites is limited, as more sites are occupied by BAX from the sample, fewer sites are left to bind BAX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BAX concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse BCL2 Associated X Protein ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between BAX and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse BCL2 Associated X Protein ELISA kit

Summary of the Assay Procedure for Mouse BCL2 Associated X Protein ELISA kit

Citations of Mouse BCL2 Associated X Protein ELISA kit

E03B0032 has been referenced in the below publications:

Effects of matrine on the proliferation and apoptosis of human medulloblastoma cell line D341.

Apoptosis in HepG2 cells induced by zinc pyrithione via mitochondrial dysfunction pathway: Involvement of zinc accumulation and oxidative stress.

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