E03B0697 Mouse Blood Urea Nitrogen ELISA kit
The Mouse Blood Urea Nitrogen ELISA kit can be used to identify samples from the mouse species. Blood Urea Nitrogen can also be called BUN.
Specifications of Mouse Blood Urea Nitrogen ELISA kit
Product Information | |
Cat. No. | E03B0697 |
Product Name | Mouse Blood Urea Nitrogen ELISA kitv |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10mmoL/L |
Sensitivity | 0.1 mmol/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 mmoL/L | 1 vial |
STANDARD B (0.5mL) | 0.5 mmoL/L | 1 vial |
STANDARD C (0.5mL) | 1.0 mmoL/L | 1 vial |
STANDARD D (0.5mL) | 2.5 mmoL/L | 1 vial |
STANDARD E (0.5mL) | 5.0 mmoL/L | 1 vial |
STANDARD F (0.5mL) | 10 mmoL/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
BUN ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-BUN antibody and an BUN-HRP conjugate. The assay sample and buffer are incubated together with BUN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BUN concentration since BUN from samples and BUN-HRP conjugate compete for the anti-BUN antibody binding site. Since the number of sites is limited, as more sites are occupied by BUN from the sample, fewer sites are left to bind BUN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BUN concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Blood Urea Nitrogen ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between BUN and analogues was observed. | |
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Summary of the Assay Procedure for Mouse Blood Urea Nitrogen ELISA kit
Citations of Mouse Blood Urea Nitrogen ELISA kit
E03B0697 has been referenced in the below publications：
Anti—Fatigue Activity of Flavonoids from Herbahouttuynia.
