E03B0452 Mouse Brain Natriuretic Peptide ELISA kit
The Mouse Brain Natriuretic Peptide ELISA kit can be used to identify samples from the mouse species. Brain Natriuretic Peptide can also be called BNP 32, Brain natriuretic peptide 32, brain natriuretic peptide, Gamma brain natriuretic peptide, Natriuretic peptide brain type, Natriuretic peptide precursor B, Natriuretic peptides B, NPPB, NPPB protein, BNP．
Product Information | |
Cat. No. | E03B0452 |
Product Name | Mouse Brain Natriuretic Peptide ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000pg/ml |
Sensitivity | 1.0pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/ml | 1 vial |
STANDARD B (0.5mL) | 50 pg/ml | 1 vial |
STANDARD C (0.5mL) | 100 pg/ml | 1 vial |
STANDARD D (0.5mL) | 250 pg/ml | 1 vial |
STANDARD E (0.5mL) | 500 pg/ml | 1 vial |
STANDARD F (0.5mL) | 1000 pg/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
BNP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-BNP antibody and an BNP-HRP conjugate. The assay sample and buffer are incubated together with BNP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BNP concentration since BNP from samples and BNP-HRP conjugate compete for the anti-BNP antibody binding site. Since the number of sites is limited, as more sites are occupied by BNP from the sample, fewer sites are left to bind BNP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BNP concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between BNP and analogues was observed. | |
E03B0452 has been referenced in the below publications：
TLR9在细菌DNA诱导心肌炎症反应中BNP的作用。
Protective Effects of PKC inhibitor Chelerythrine on Cardiotoxicity induced by Sunitinib.
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