E03C0096 Mouse Caspase 9 ELISA kit
The Mouse Caspase 9 ELISA kit can be used to identify samples from the mouse species. Caspase 9 can also be called CASP9, MCH6, APAF3, APAF-3, PPP1R56, ICE-LAP6, caspase 9.
Specifications of Mouse Caspase 9 ELISA kit
Product Information | |
Cat. No. | E03C0096 |
Product Name | Mouse Caspase 9 ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25ng/ml |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/ml | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/ml | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/ml | 1 vial |
STANDARD E (0.5mL) | 10 ng/ml | 1 vial |
STANDARD F (0.5mL) | 25 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Caspase 9 ELISA kit uses an anti-Caspase 9 antibody and an Caspase 9-HRP conjugate in a competitive enzyme immunoassay method. Caspase 9-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Caspase 9 antibody binding site between Caspase 9 from samples and Caspase 9-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Caspase 9. Since the number of sites is limited, as more sites are occupied by Caspase 9 from the sample, fewer sites are left to bind Caspase 9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Caspase 9 concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Caspase 9 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Caspase 9 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Caspase 9 ELISA kit
Citations of Mouse Caspase 9 ELISA kit
E03C0096 has been referenced in the below publications：
Effects of matrine on the proliferation and apoptosis of human medulloblastoma cell line D341.
