E03C0745 Mouse Cholesterol ELISA kit
The Mouse Cholesterol ELISA kit can be used to identify samples from the mouse species. Cholesterol can also be called Cholesterin, Cholesteryl alcohol, and CH.
Specifications of Mouse Cholesterol ELISA kit
Product Information | |
Cat. No. | E03C0745 |
Product Name | Mouse Cholesterol ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10mmoL/L |
Sensitivity | 0.1 mmol/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 mmoL/L | 1 vial |
STANDARD B (0.5mL) | 0.5 mmoL/L | 1 vial |
STANDARD C (0.5mL) | 1.0 mmoL/L | 1 vial |
STANDARD D (0.5mL) | 2.5 mmoL/L | 1 vial |
STANDARD E (0.5mL) | 5.0 mmoL/L | 1 vial |
STANDARD F (0.5mL) | 10 mmoL/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CH ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CH antibody and an CH-HRP conjugate. The assay sample and buffer are incubated together with CH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CH concentration since CH from samples and CH-HRP conjugate compete for the anti-CH antibody binding site. Since the number of sites is limited, as more sites are occupied by CH from the sample, fewer sites are left to bind CH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CH concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Cholesterol ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CH and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Cholesterol ELISA kit
Citations of Mouse Cholesterol ELISA kit
E03C0745 has been referenced in the below publications：
The research on antioxidant effect and active function of Tyr-Ala di-peptide.
种具有降血脂降血糖活性的组合物及其制备方法与应用。
