E03C0777 Mouse Ciliary Neurotrophic Factor ELISA kit
The Mouse Ciliary Neurotrophic Factor ELISA kit can be used to identify samples from the mouse species. Ciliary Neurotrophic Factor can also be called CNTF, Hciliary neurotrophic factor.
Specifications of Mouse Ciliary Neurotrophic Factor ELISA kit
Product Information | |
Cat. No. | E03C0777 |
Product Name | Mouse Ciliary Neurotrophic Factor ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000pg/ml |
Sensitivity | 1.0pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 250 pg/mL | 1 vial |
STANDARD C (0.5mL) | 500 pg/mL | 1 vial |
STANDARD D (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD E (0.5mL) | 2500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 5000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CNTF ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CNTF antibody and an CNTF-HRP conjugate. The assay sample and buffer are incubated together with CNTF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CNTF concentration since CNTF from samples and CNTF-HRP conjugate compete for the anti-CNTF antibody binding site. Since the number of sites is limited, as more sites are occupied by CNTF from the sample, fewer sites are left to bind CNTF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNTF concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Ciliary Neurotrophic Factor ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CNTF and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Ciliary Neurotrophic Factor ELISA kit
Citations of Mouse Ciliary Neurotrophic Factor ELISA kit
E03C0777 has been referenced in the below publications：
Restoration of glucose metabolism in leptin-resistant mouse hearts after acute myocardial infarction through the activation of survival kinase pathways.
