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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Coiled Coil Myosin Like BCL2 Interacting Protein ELISA kit (E03C1217)

E03C1217 Mouse Coiled Coil Myosin Like BCL2 Interacting Protein ELISA kit

The Mouse Coiled Coil Myosin Like BCL2 Interacting Protein ELISA kit can be used to identify samples from the mouse species. Coiled Coil Myosin Like BCL2 Interacting Protein can also be called  BECN1, ATG6, VPS30, beclin1, and beclin 1.

Products

Specifications of Mouse Coiled Coil Myosin Like BCL2 Interacting Protein ELISA kit

Product Information

Cat. No.

E03C1217

Product Name

Mouse Coiled Coil Myosin Like BCL2 Interacting Protein ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

0.5-10ng/ml

Sensitivity

0.1ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml1 vial

STANDARD B (0.5mL)

0.5 ng/ml

1 vial

STANDARD C (0.5mL)

1.0 ng/ml

1 vial

STANDARD D (0.5mL)

2.5 ng/ml

1 vial

STANDARD E (0.5mL)

5.0 ng/ml

1 vial

STANDARD F (0.5mL)

10 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

BECN1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-BECN1 antibody and a BECN1-HRP conjugate. The assay sample and buffer are incubated together with BECN1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BECN1 concentration since BECN1 from samples and BECN1-HRP conjugate compete for the anti-BECN1 antibody binding site. Since the number of sites is limited, as more sites are occupied by BECN1 from the sample, fewer sites are left to bind BECN1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BECN1 concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Coiled Coil Myosin Like BCL2 Interacting Protein ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between BECN1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Coiled Coil Myosin Like BCL2 Interacting Protein ELISA kit

Summary of the Assay Procedure for Mouse Coiled Coil Myosin Like BCL2 Interacting Protein ELISA kit

Citations of Mouse Coiled Coil Myosin Like BCL2 Interacting Protein ELISA kit

E03C1217 has been referenced in the below publications:

The suppressed autophagy induced by carbon disulfide could be rescued by N-carbamoyl glutamate during the window of embryo implantation in mice.

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