Mouse Coiled Coil Myosin Like BCL2 Interacting Protein ELISA kit (E03C1217)

BlueGene Biotech Mouse Coiled Coil Myosin Like BCL2 Interacting Protein ELISA kit (E03C1217)

Cancer

Microbiology

Epigenetics and Nuclear Signaling

E03C1217 Mouse Coiled Coil Myosin Like BCL2 Interacting Protein ELISA kit

The Mouse Coiled Coil Myosin Like BCL2 Interacting Protein ELISA kit can be used to identify samples from the mouse species. Coiled Coil Myosin Like BCL2 Interacting Protein can also be called BECN1, ATG6, VPS30, beclin1, and beclin 1.

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Specifications of Mouse Coiled Coil Myosin Like BCL2 Interacting Protein ELISA kit

Product Information
Cat. No.	E03C1217
Product Name	Mouse Coiled Coil Myosin Like BCL2 Interacting Protein ELISA kit
Species	Mouse
Product Size	48 Tests / 96 Tests
Concentration	0.5-10ng/ml
Sensitivity	0.1ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/ml	1 vial
STANDARD B (0.5mL)	0.5 ng/ml	1 vial
STANDARD C (0.5mL)	1.0 ng/ml	1 vial
STANDARD D (0.5mL)	2.5 ng/ml	1 vial
STANDARD E (0.5mL)	5.0 ng/ml	1 vial
STANDARD F (0.5mL)	10 ng/ml	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

BECN1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-BECN1 antibody and a BECN1-HRP conjugate. The assay sample and buffer are incubated together with BECN1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BECN1 concentration since BECN1 from samples and BECN1-HRP conjugate compete for the anti-BECN1 antibody binding site. Since the number of sites is limited, as more sites are occupied by BECN1 from the sample, fewer sites are left to bind BECN1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BECN1 concentration in each sample is interpolated from this standard curve.

Quality Control on Mouse Coiled Coil Myosin Like BCL2 Interacting Protein ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between BECN1 and analogues was observed.

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Summary of the Assay Procedure for Mouse Coiled Coil Myosin Like BCL2 Interacting Protein ELISA kit

Citations of Mouse Coiled Coil Myosin Like BCL2 Interacting Protein ELISA kit

E03C1217 has been referenced in the below publications：

The suppressed autophagy induced by carbon disulfide could be rescued by N-carbamoyl glutamate during the window of embryo implantation in mice.