E03C0047 Mouse Collagen Type IV ELISA kit
The Mouse Collagen Type IV ELISA kit can be used to identify samples from the mouse species. Collagen Type IV can also be called ColIV, Col4, or COL4.
Specifications of Mouse Collagen Type IV ELISA kit
Product Information | |
Cat. No. | E03C0047 |
Product Name | Mouse Collagen Type IV ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000ng/mL |
Sensitivity | 1.0ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 50 ng/mL | 1 vial |
STANDARD C (0.5mL) | 100 ng/mL | 1 vial |
STANDARD D (0.5mL) | 250 ng/mL | 1 vial |
STANDARD E (0.5mL) | 500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
COL4 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-COL4 antibody and an COL4-HRP conjugate. The assay sample and buffer are incubated together with COL4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COL4 concentration since COL4 from samples and COL4-HRP conjugate compete for the anti-COL4 antibody binding site. Since the number of sites is limited, as more sites are occupied by COL4 from the sample, fewer sites are left to bind COL4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COL4 concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Collagen Type IV ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between COL4 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Collagen Type IV ELISA kit
Citations of Mouse Collagen Type IV ELISA kit
E03C0047 has been referenced in the below publications：
The protective effect of 36 amino acid polypeptide fragment to CCl 4-induced liver fibrosis and its mechanism.
