E03P0031 Mouse Corticotropin Releasing Hormone ELISA kit
The Mouse Corticotropin Releasing Hormone ELISA kit can be used to identify samples from the mouse species. Corticotropin Releasing Hormone can also be called CRF, CRH1．
Specifications of Mouse Corticotropin Releasing Hormone ELISA kit
Product Information | |
Cat. No. | E03P0031 |
Product Name | Mouse Corticotropin Releasing Hormone ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000pg/ml |
Sensitivity | 1.0pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/ml | 1 vial |
STANDARD B (0.5mL) | 50 pg/ml | 1 vial |
STANDARD C (0.5mL) | 100 pg/ml | 1 vial |
STANDARD D (0.5mL) | 250 pg/ml | 1 vial |
STANDARD E (0.5mL) | 500 pg/ml | 1 vial |
STANDARD F (0.5mL) | 1000 pg/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CRH ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CRH antibody and an CRH-HRP conjugate. The assay sample and buffer are incubated together with CRH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CRH concentration since CRH from samples and CRH-HRP conjugate compete for the anti-CRH antibody binding site. Since the number of sites is limited, as more sites are occupied by CRH from the sample, fewer sites are left to bind CRH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CRH concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Corticotropin Releasing Hormone ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CRH and analogues was observed. | |
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Summary of the Assay Procedure for Mouse Corticotropin Releasing Hormone ELISA kit
Citations of Mouse Corticotropin Releasing Hormone ELISA kit
E03P0031 has been referenced in the below publications：
Glucocorticoids impair oocyte developmental potential by triggering apoptosis of ovarian cells via activating the Fas system.
