E03C0629 Mouse Creatinine ELISA kit
The Mouse Creatinine ELISA kit can be used to identify samples from the mouse species. Creatinine can also be called 2-Amino-1-methyl-5H-imidazol-4-, CREA.
Specifications of Mouse Creatinine ELISA kit
Product Information | |
Cat. No. | E03C0629 |
Product Name | Mouse Creatinine ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500μmol/L |
Sensitivity | 1.0μmol/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 μmol/L | 1 vial |
STANDARD B (0.5mL) | 25 μmol/L | 1 vial |
STANDARD C (0.5mL) | 50 μmol/L | 1 vial |
STANDARD D (0.5mL) | 100 μmol/L | 1 vial |
STANDARD E (0.5mL) | 250 μmol/L | 1 vial |
STANDARD F (0.5mL) | 500 μmol/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CREA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CREA antibody and an CREA-HRP conjugate. The assay sample and buffer are incubated together with CREA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CREA concentration since CREA from samples and CREA-HRP conjugate compete for the anti-CREA antibody binding site. Since the number of sites is limited, as more sites are occupied by CREA from the sample, fewer sites are left to bind CREA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CREA concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Creatinine ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CREA and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Creatinine ELISA kit
Citations of Mouse Creatinine ELISA kit
E03C0629 has been referenced in the below publications：
Fraxetin inhibits the growth of colon adenocarcinoma cells via the Janus kinase 2/signal transducer and activator of transcription 3 signalling pathway.
