E03C0027 Mouse Cyclic Adenosine Monophosphate ELISA kit
The Mouse Cyclic Adenosine Monophosphate ELISA kit can be used to identify samples from the mouse species. Cyclic Adenosine Monophosphate can also be called cAMP, cyclic AMP, 3',5'-cyclic adenosine monophosphate.
Product Information | |
Cat. No. | E03C0027 |
Product Name | Mouse Cyclic Adenosine Monophosphate ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000pmol/ml |
Sensitivity | 1.0pmol/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 pmol/mL | 1 vial |
STANDARD B (0.5mL) | 50 pmol/mL | 1 vial |
STANDARD C (0.5mL) | 100 pmol/mL | 1 vial |
STANDARD D (0.5mL) | 250 pmol/mL | 1 vial |
STANDARD E (0.5mL) | 500 pmol/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pmol/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
cAMP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for cAMP. Standards or samples are then added to the microtiter plate wells and cAMP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of cAMP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for cAMP are added to each well to “sandwich” the cAMP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain cAMP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The cAMP concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between cAMP and analogues was observed. | |
E03C0027 has been referenced in the below publications：
Triglyceride with medium-chain fatty acids increases the activity and expression of hormone-sensitive lipase in white adipose tissue of C57BL/6J mice.
Effect of medium chain triglycerides on brown adipose tissue and its β3 adrenergic receptors in mice with obesity induced by high fat diet.
Effect of common edible oils on lipid metabolism and its mechanism in obese mice induced by a high fat diet.
Effect of medium chain triglycerides on brown adipose tissue in obese mice.
The Phosphodiesterase-4 Inhibitor Roflumilast, a Potential Treatment for the Comorbidity of Memory Loss and Depression in Alzheimer’s Disease:A Preclinical Study in APP/PS1 Transgenic Mice.
The Phosphodiesterase-4 Inhibitor Roflumilast, a Potential Treatment for the Comorbidity of Memory Loss and Depression in Alzheimer’s Disease: A Preclinical Study in APP/PS1 Transgenic Mice.
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