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BlueGene Biotech Mouse Cyclic Adenosine Monophosphate ELISA kit (E03C0027)

E03C0027 Mouse Cyclic Adenosine Monophosphate ELISA kit

The Mouse Cyclic Adenosine Monophosphate ELISA kit can be used to identify samples from the mouse species. Cyclic Adenosine Monophosphate can also be called cAMP, cyclic AMP, 3',5'-cyclic adenosine monophosphate.

Products

Specifications of Mouse Cyclic Adenosine Monophosphate ELISA kit

Product Information

Cat. No.

E03C0027

Product Name

Mouse Cyclic Adenosine Monophosphate ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

50-1000pmol/ml

Sensitivity

1.0pmol/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 pmol/mL

1 vial

STANDARD B (0.5mL)

50 pmol/mL

1 vial

STANDARD C (0.5mL)

100 pmol/mL

1 vial

STANDARD D (0.5mL)

250 pmol/mL

1 vial

STANDARD E (0.5mL)

500 pmol/mL

1 vial

STANDARD F (0.5mL)

1000 pmol/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

cAMP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for cAMP. Standards or samples are then added to the microtiter plate wells and cAMP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of cAMP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for cAMP are added to each well to sandwichthe cAMP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain cAMP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The cAMP concentration in each sample is interpolated from this standard curve.


Quality Control on Mouse Cyclic Adenosine Monophosphate ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between cAMP and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Cyclic Adenosine Monophosphate ELISA kit

Summary of the Assay Procedure for Mouse Cyclic Adenosine Monophosphate ELISA kit

Citations of Mouse Cyclic Adenosine Monophosphate ELISA kit

E03C0027 has been referenced in the below publications:

Triglyceride with medium-chain fatty acids increases the activity and expression of hormone-sensitive lipase in white adipose tissue of C57BL/6J mice.

Effect of medium chain triglycerides on brown adipose tissue and its β3 adrenergic receptors in mice with obesity induced by high fat diet.

Effect of common edible oils on lipid metabolism and its mechanism in obese mice induced by a high fat diet.

Effect of medium chain triglycerides on brown adipose tissue in obese mice.

The Phosphodiesterase-4 Inhibitor Roflumilast, a Potential Treatment for the Comorbidity of Memory Loss and Depression in Alzheimer’s Disease:A Preclinical Study in APP/PS1 Transgenic Mice.

The Phosphodiesterase-4 Inhibitor Roflumilast, a Potential Treatment for the Comorbidity of Memory Loss and Depression in Alzheimer’s Disease: A Preclinical Study in APP/PS1 Transgenic Mice.


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