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BlueGene Biotech Mouse Deoxypyridinoline ELISA kit (E03D0033)

E03D0033 Mouse Deoxypyridinoline ELISA kit

The Mouse Deoxypyridinoline ELISA kit can be used to identify samples from the mouse species. Deoxypyridinoline can also be called  DPD, DPYD, DHPDHase, Dihydrothymine dehydrogenase, and Dihydrouracil dehydrogenase.

Products

Specifications of Mouse Deoxypyridinoline ELISA kit

Product Information

Cat. No.

E03D0033

Product Name

Mouse Deoxypyridinoline ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

25-500nmol/L

Sensitivity

1.0nmol/L

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 nmol/L1 vial

STANDARD B (0.5mL)

25 nmol/L

1 vial

STANDARD C (0.5mL)

50 nmol/L

1 vial

STANDARD D (0.5mL)

100 nmol/L

1 vial

STANDARD E (0.5mL)

250 nmol/L

1 vial

STANDARD F (0.5mL)

500 nmol/L

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

DPD ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-DPD antibody and an DPD-HRP conjugate. The assay sample and buffer are incubated together with DPD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DPD concentration since DPD from samples and DPD-HRP conjugate compete for the anti-DPD antibody binding site. Since the number of sites is limited, as more sites are occupied by DPD from the sample, fewer sites are left to bind DPD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DPD concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Deoxypyridinoline ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between DPD and analogues was observed.


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Summary of the Assay Procedure for Mouse Deoxypyridinoline ELISA kit

Summary of the Assay Procedure for Mouse Deoxypyridinoline ELISA kit

Citations of Mouse Deoxypyridinoline ELISA kit

E03D0033 has been referenced in the below publications:

Metabolism and mechanism of articular chondrocytes in young Wistar rats exposed to deoxynivalenol.

Contribution of Inflammation and Bone Destruction to Pain in Arthritis: A Study in Murine Glucose-6-Phosphate Isomerase-Induced Arthritis.




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