E03E0023 Mouse E2 Estradiol ELISA kit
The Mouse E2 Estradiol ELISA kit can be used to identify samples from the mouse species. E2 Estradiol can also be called Estradiol, E2 estradiol, 17B-Estradiol, E2, Oestradiol, Beta-Estradiol．
Specifications of Mouse E2 Estradiol ELISA kit
Product Information | |
Cat. No. | E03E0023 |
Product Name | Mouse E2 Estradiol ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000pg/ml |
Sensitivity | 1.0pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/ml | 1 vial |
STANDARD B (0.5mL) | 50 pg/ml | 1 vial |
STANDARD C (0.5mL) | 100 pg/ml | 1 vial |
STANDARD D (0.5mL) | 250 pg/ml | 1 vial |
STANDARD E (0.5mL) | 500 pg/ml | 1 vial |
STANDARD F (0.5mL) | 1000 pg/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
E2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-E2 antibody and an E2-HRP conjugate. The assay sample and buffer are incubated together with E2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the E2 concentration since E2 from samples and E2-HRP conjugate compete for the anti-E2 antibody binding site. Since the number of sites is limited, as more sites are occupied by E2 from the sample, fewer sites are left to bind E2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The E2 concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse E2 Estradiol ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between E2 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse E2 Estradiol ELISA kit
Citations of Mouse E2 Estradiol ELISA kit
E03E0023 has been referenced in the below publications：
Effect of follicle stimulating hormone on cryopreserved sheep ovarian tissue xenotransplantation.
Effects of carbon disulfide exposure during peri-implantation onestrogen receptor-α expression in uterus and serum level of estrogen in pregnant mice.
Different kinds of moxa smoke effects on physical of the pregnant mice and the growth and development of the fetal mice.
Down-regulation of uterine LIF expression induced by the hormonal level disorder causes embryo implantation loss after mice exposed to carbon disulfide at peri-implantation.
Inhibitory activities of some traditional Chinese herbs against testosterone 5α-reductase and effects of Cacumen platycladi on hair re-growth in testosterone-treated mice.
Long non‑coding RNA NEAT1 promotes mouse granulosa cell proliferation and estradiol synthesis by sponging miR‑874‑3p.
