En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Endothelial Lipase ELISA kit (E03E0137)

E03E0137 Mouse Endothelial Lipase ELISA kit

The Mouse Endothelial Lipase ELISA kit can be used to identify samples from the mouse species. Endothelial Lipase can also be called LIPG, EL, EDL, Endothelial Lipase, and Endothelial cell-derived lipase.

Products

Specifications of Mouse Endothelial Lipase ELISA kit

Product Information

Cat. No.

E03E0137

Product Name

Mouse Endothelial Lipase ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

10-250 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

10 ng/mL

1 vial

STANDARD C (0.5mL)

25 ng/mL

1 vial

STANDARD D (0.5mL)

50 ng/mL

1 vial

STANDARD E (0.5mL)

100 ng/mL

1 vial

STANDARD F (0.5mL)

250 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

EL ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-EL antibody and an EL-HRP conjugate. The assay sample and buffer are incubated together with EL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EL concentration since EL from samples and EL-HRP conjugate compete for the anti-EL antibody binding site. Since the number of sites is limited, as more sites are occupied by EL from the sample, fewer sites are left to bind EL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EL concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Endothelial Lipase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between EL and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Endothelial Lipase ELISA kit

Summary of the Assay Procedure for Mouse Endothelial Lipase ELISA kit

Citations of Mouse Endothelial Lipase ELISA kit

E03E0137 has been referenced in the below publications:

Effect of common edible oils on lipid metabolism and its mechanism in obese mice induced by a high fat diet.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.