E03E0137 Mouse Endothelial Lipase ELISA kit
The Mouse Endothelial Lipase ELISA kit can be used to identify samples from the mouse species. Endothelial Lipase can also be called LIPG, EL, EDL, Endothelial Lipase, and Endothelial cell-derived lipase.
Specifications of Mouse Endothelial Lipase ELISA kit
Product Information | |
Cat. No. | E03E0137 |
Product Name | Mouse Endothelial Lipase ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 10-250 ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 10 ng/mL | 1 vial |
STANDARD C (0.5mL) | 25 ng/mL | 1 vial |
STANDARD D (0.5mL) | 50 ng/mL | 1 vial |
STANDARD E (0.5mL) | 100 ng/mL | 1 vial |
STANDARD F (0.5mL) | 250 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
EL ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-EL antibody and an EL-HRP conjugate. The assay sample and buffer are incubated together with EL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EL concentration since EL from samples and EL-HRP conjugate compete for the anti-EL antibody binding site. Since the number of sites is limited, as more sites are occupied by EL from the sample, fewer sites are left to bind EL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EL concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Endothelial Lipase ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between EL and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Endothelial Lipase ELISA kit
Citations of Mouse Endothelial Lipase ELISA kit
E03E0137 has been referenced in the below publications：
Effect of common edible oils on lipid metabolism and its mechanism in obese mice induced by a high fat diet.
