Mouse Epidermal Growth Factor Receptor ELISA kit (E03E0129)

BlueGene Biotech Mouse Epidermal Growth Factor Receptor ELISA kit (E03E0129)

E03E0129 Mouse Epidermal Growth Factor Receptor ELISA kit

The Mouse Epidermal Growth Factor Receptor ELISA kit can be used to identify samples from the mouse species. Epidermal Growth Factor Receptor can also be called ErbB-1, EGFR, ERBB, ERBB1, HER1, NISBD2, PIG61, mENA, epidermal growth factor receptor, Genes, erbB-1, ERRP．

Products

Specifications of Mouse Epidermal Growth Factor Receptor ELISA kit

Product Information
Cat. No.	E03E0129
Product Name	Mouse Epidermal Growth Factor Receptor ELISA kit
Species	Mouse
Product Size	48 Tests / 96 Tests
Concentration	0.5-10ng/ml
Sensitivity	0.1ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/ml	1 vial
STANDARD B (0.5mL)	0.5 ng/ml	1 vial
STANDARD C (0.5mL)	1.0 ng/ml	1 vial
STANDARD D (0.5mL)	2.5 ng/ml	1 vial
STANDARD E (0.5mL)	5.0ng/ml	1 vial
STANDARD F (0.5mL)	10 ng/ml	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

EGFR ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-EGFR antibody and a EGFR-HRP conjugate. The assay sample and buffer are incubated together with EGFR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EGFR concentration since EGFR from samples and EGFR-HRP conjugate compete for the anti-EGFR antibody binding site. Since the number of sites is limited, as more sites are occupied by EGFR from the sample, fewer sites are left to bind EGFR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EGFR concentration in each sample is interpolated from this standard curve.

Quality Control on Mouse Epidermal Growth Factor Receptor ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between EGFR and analogues was observed.