Mouse Fibronectin ELISA kit (E03F0004) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Mouse Fibronectin ELISA kit (E03F0004)

E03F0004 Mouse Fibronectin ELISA kit

The Mouse Fibronectin ELISA kit can be used to identify samples from the mouse species. Fibronectin can also be called CIG, Cold insoluble globulin, ED B, ED-B, fibronectin 1, FINC, FN 1 FN1, FNZ, GFND, GFND2, LETS, Migration stimulating factor, MSF, Transformation sensitive protein, Fibronectin, Cold-insoluble globulin, FN．

Products

Specifications of Mouse Fibronectin ELISA kit

Product Information
Cat. No.	E03F0004
Product Name	Mouse Fibronectin ELISA kit
Species	Mouse
Product Size	48 Tests / 96 Tests
Concentration	0.5-10ng/ml
Sensitivity	0.1ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/ml	1 vial
STANDARD B (0.5mL)	0.5 ng/ml	1 vial
STANDARD C (0.5mL)	1.0 ng/ml	1 vial
STANDARD D (0.5mL)	2.5 ng/ml	1 vial
STANDARD E (0.5mL)	5.0ng/ml	1 vial
STANDARD F (0.5mL)	10 ng/ml	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

FN ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-FN antibody and a FN-HRP conjugate. The assay sample and buffer are incubated together with FN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FN concentration since FN from samples and FN-HRP conjugate compete for the anti-FN antibody binding site. Since the number of sites is limited, as more sites are occupied by FN from the sample, fewer sites are left to bind FN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FN concentration in each sample is interpolated from this standard curve.

Quality Control on Mouse Fibronectin ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between FN and analogues was observed.