En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Fibronectin ELISA kit (E03F0004)

E03F0004 Mouse Fibronectin ELISA kit

The Mouse Fibronectin ELISA kit can be used to identify samples from the mouse species. Fibronectin can also be called CIG, Cold insoluble globulin, ED B, ED-B, fibronectin 1, FINC, FN 1 FN1, FNZ, GFND, GFND2, LETS, Migration stimulating factor, MSF, Transformation sensitive protein, Fibronectin, Cold-insoluble globulin, FN.

Products

Specifications of Mouse Fibronectin ELISA kit

Product Information

Cat. No.

E03F0004

Product Name

Mouse Fibronectin ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

0.5-10ng/ml

Sensitivity

0.1ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml1 vial

STANDARD B (0.5mL)

0.5 ng/ml

1 vial

STANDARD C (0.5mL)

1.0 ng/ml

1 vial

STANDARD D (0.5mL)

2.5 ng/ml

1 vial

STANDARD E (0.5mL)

5.0ng/ml

1 vial

STANDARD F (0.5mL)

10 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

FN ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-FN antibody and a FN-HRP conjugate. The assay sample and buffer are incubated together with FN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FN concentration since FN from samples and FN-HRP conjugate compete for the anti-FN antibody binding site. Since the number of sites is limited, as more sites are occupied by FN from the sample, fewer sites are left to bind FN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FN concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Fibronectin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between FN and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Fibronectin ELISA kit

Summary of the Assay Procedure for Mouse Fibronectin ELISA kit

Citations of Mouse Fibronectin ELISA kit

E03F0004 has been referenced in the below publications:

Study on the regulation and mechanism that Mycobacterium Tuberculosis reacts at the iron metabolism of infected Mouse Alveolar macrophages.


Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.