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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse FOS Like Antigen 1 ELISA kit (E03F0013)

E03F0013 Mouse FOS Like Antigen 1 ELISA kit

The Mouse FOS Like Antigen 1 ELISA kit can be used to identify samples from the mouse species. FOS Like Antigen 1 can also be called FOSL1, Fra-1.

Products

Specifications of Mouse FOS Like Antigen 1 ELISA kit

Product Information

Cat. No.

E03F0013

Product Name

Mouse FOS Like Antigen 1 ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

5-100ng/ml

Sensitivity

1.0ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml

1 vial

STANDARD B (0.5mL)

5.0 ng/ml

1 vial

STANDARD C (0.5mL)

10 ng/ml

1 vial

STANDARD D (0.5mL)

2.5 ng/ml

1 vial

STANDARD E (0.5mL)

50 ng/ml

1 vial

STANDARD F (0.5mL)

100 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

FOSL1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-FOSL1 antibody and a FOSL1-HRP conjugate. The assay sample and buffer are incubated together with FOSL1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FOSL1 concentration since FOSL1 from samples and FOSL1-HRP conjugate compete for the anti-FOSL1 antibody binding site. Since the number of sites is limited, as more sites are occupied by FOSL1 from the sample, fewer sites are left to bind FOSL1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FOSL1 concentration in each sample is interpolated from this standard curve.


Quality Control on Mouse FOS Like Antigen 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between FOSL1 and analogues was observed.


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Summary of the Assay Procedure for Mouse FOS Like Antigen 1 ELISA kit

Summary of the Assay Procedure for Mouse FOS Like Antigen 1 ELISA kit

Citations of Mouse FOS Like Antigen 1 ELISA kit

E03F0013 has been referenced in the below publications:

Qilongtian ameliorate bleomycin‑induced pulmonary fibrosis in mice via inhibiting IL‑17 signal pathway.

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