E03F0002 Mouse Free Fatty Acid ELISA kit
The Mouse Free Fatty Acid ELISA kit can be used to identify samples from the mouse species. Free Fatty Acid can also be called NEFA, nonestesterified fatty acid, free fatty acid, or FFA.
Specifications of Mouse Free Fatty Acid ELISA kit
Product Information | |
Cat. No. | E03F0002 |
Product Name | Mouse Free Fatty Acid ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000ug/ml |
Sensitivity | 1.0ug/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/ml | 1 vial |
STANDARD B (0.5mL) | 50 ug/ml | 1 vial |
STANDARD C (0.5mL) | 100 ug/ml | 1 vial |
STANDARD D (0.5mL) | 250 ug/ml | 1 vial |
STANDARD E (0.5mL) | 500 ug/ml | 1 vial |
STANDARD F (0.5mL) | 1000 ug/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
FFA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-FFA antibody and an FFA-HRP conjugate. The assay sample and buffer are incubated together with FFA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FFA concentration since FFA from samples and FFA-HRP conjugate compete for the anti-FFA antibody binding site. Since the number of sites is limited, as more sites are occupied by FFA from the sample, fewer sites are left to bind FFA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FFA concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Free Fatty Acid ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between FFA and analogues was observed. | |
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Summary of the Assay Procedure for Mouse Free Fatty Acid ELISA kit
Citations of Mouse Free Fatty Acid ELISA kit
E03F0002 has been referenced in the below publications：
Triglyceride with medium-chain fatty acids increases the activity and expression of hormone-sensitive lipase in white adipose tissue of C57BL/6J mice.
Western diet induces colonic nitrergic myenteric neuropathy and dysmotility in mice via saturated fatty acid- and lipopolysaccharide-induced TLR4 signalling.
