E03G0281 Mouse Gastrin ELISA kit
The Mouse Gastrin ELISA kit can be used to identify samples from the mouse species. Gastrin can also be called GAST, GAS, gastrin, GT.
Specifications of Mouse Gastrin ELISA kit
Product Information | |
Cat. No. | E03G0281 |
Product Name | Mouse Gastrin ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500pg/mL |
Sensitivity | 1.0pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/ml | 1 vial |
STANDARD B (0.5mL) | 25 pg/ml | 1 vial |
STANDARD C (0.5mL) | 50 pg/ml | 1 vial |
STANDARD D (0.5mL) | 100 pg/ml | 1 vial |
STANDARD E (0.5mL) | 250 pg/ml | 1 vial |
STANDARD F (0.5mL) | 500 pg/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
GT ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GT antibody and an GT-HRP conjugate. The assay sample and buffer are incubated together with GT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GT concentration since GT from samples and GT-HRP conjugate compete for the anti-GT antibody binding site. Since the number of sites is limited, as more sites are occupied by GT from the sample, fewer sites are left to bind GT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GT concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Gastrin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between GT and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Gastrin ELISA kit
Citations of Mouse Gastrin ELISA kit
E03G0281has been referenced in the below publications：
Changes in Small Intestinal Motility and Related Hormones by Acupuncture Stimulation at Zusanli (ST 36) in Mice.
