E03G0369 Mouse Glutathione Peroxidase ELISA kit
The Mouse Glutathione Peroxidase ELISA kit can be used to identify samples from the mouse species. Glutathione Peroxidase can also be called GPx, GSHPx, GSH Px.
Cyanidin-3-O-glucoside protects the brain and improves cognitive function in APPswe/ PS1ΔE9 transgenic mice model.
Product Information | |
Cat. No. | E03G0369 |
Product Name | Mouse Glutathione Peroxidase ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50ng/ml |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/ml | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/ml | 1 vial |
STANDARD D (0.5mL) | 10 ng/ml | 1 vial |
STANDARD E (0.5mL) | 25 ng/ml | 1 vial |
STANDARD F (0.5mL) | 50 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
GSH Px ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GSH Px antibody and an GSH Px-HRP conjugate. The assay sample and buffer are incubated together with GSH Px-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GSH Px concentration since GSH Px from samples and GSH Px-HRP conjugate compete for the anti-GSH Px antibody binding site. Since the number of sites is limited, as more sites are occupied by GSH Px from the sample, fewer sites are left to bind GSH Px-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GSH Px concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between GSH Px and analogues was observed. | |
E03G0369 has been referenced in the below publications：
Ameliorative effects of black ginseng on nonalcoholic fatty liver disease in free fatty acideinduced HepG2 cells and high-fat/highfructose diet-fed mice.
Lonicera caerulea Extract Attenuates Non-Alcoholic Fatty Liver Disease in Free Fatty Acid-Induced HepG2 Hepatocytes and in High Fat Diet-Fed Mice.
Stevia (Stevia rebaudiana) extract ameliorates insulin resistance by regulating mitochondrial function and oxidative stress in the skeletal muscle of db/db mice.
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