E03G0238 Mouse Glutathione Reductase ELISA kit
The Mouse Glutathione Reductase ELISA kit can be used to identify samples from the mouse species. Glutathione Reductase can also be called GSR, GR, GSRD, HEL-75, HEL-S-122m, glutathione reductase, glutathione-disulfide reductase.
Product Information | |
Cat. No. | E03G0238 |
Product Name | Mouse Glutathione Reductase ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25ng/ml |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/ml | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/ml | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/ml | 1 vial |
STANDARD E (0.5mL) | 10 ng/ml | 1 vial |
STANDARD F (0.5mL) | 25 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
GR ELISA kit uses an anti-GR antibody and an GR-HRP conjugate in a competitive enzyme immunoassay method. GR-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GR antibody binding site between GR from samples and GR-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GR. Since the number of sites is limited, as more sites are occupied by GR from the sample, fewer sites are left to bind GR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GR concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between GR and analogues was observed. | |
E03G0238 has been referenced in the below publications：
Alterations in epididymal proteomics and antioxidant activity of mice exposed to fluoride.
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