Mouse Glycogen Synthase Kinase 3 Alpha ELISA kit (E03G0264)

BlueGene Biotech Mouse Glycogen Synthase Kinase 3 Alpha ELISA kit (E03G0264)

E03G0264 Mouse Glycogen Synthase Kinase 3 Alpha ELISA kit

The Mouse Glycogen Synthase Kinase 3 Alpha ELISA kit can be used to identify samples from the mouse species. Glycogen Synthase Kinase 3 Alpha can also be called GSK3a, GSK3A, Gsk3a, glycogen synthase kinase 3 alpha, Serine/threonine-protein kinase GSK3A.

Products

Specifications of Mouse Glycogen Synthase Kinase 3 Alpha ELISA kit

Product Information
Cat. No.	E03G0264
Product Name	Mouse Glycogen Synthase Kinase 3 Alpha ELISA kit
Species	Mouse
Product Size	48 Tests / 96 Tests
Concentration	1.0-25ng/ml
Sensitivity	0.1ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/ml	1 vial
STANDARD B (0.5mL)	1.0 ng/ml	1 vial
STANDARD C (0.5mL)	2.5 ng/ml	1 vial
STANDARD D (0.5mL)	5.0 ng/ml	1 vial
STANDARD E (0.5mL)	10 ng/ml	1 vial
STANDARD F (0.5mL)	25 ng/ml	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

GSK3a ELISA kit uses an anti-GSK3a antibody and an GSK3a-HRP conjugate in a competitive enzyme immunoassay method. GSK3a-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GSK3a antibody binding site between GSK3a from samples and GSK3a-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GSK3a. Since the number of sites is limited, as more sites are occupied by GSK3a from the sample, fewer sites are left to bind GSK3a-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GSK3a concentration in each sample is interpolated from this standard curve.

Quality Control on Mouse Glycogen Synthase Kinase 3 Alpha ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between GSK3a and analogues was observed.