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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Glycogen Synthase Kinase 3 Alpha ELISA kit (E03G0264)

E03G0264 Mouse Glycogen Synthase Kinase 3 Alpha ELISA kit

The Mouse Glycogen Synthase Kinase 3 Alpha ELISA kit can be used to identify samples from the mouse species. Glycogen Synthase Kinase 3 Alpha can also be called GSK3a, GSK3A, Gsk3a, glycogen synthase kinase 3 alpha, Serine/threonine-protein kinase GSK3A.

Products

Specifications of Mouse Glycogen Synthase Kinase 3 Alpha ELISA kit

Product Information

Cat. No.

E03G0264

Product Name

Mouse Glycogen Synthase Kinase 3 Alpha ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

1.0-25ng/ml

Sensitivity

0.1ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml1 vial

STANDARD B (0.5mL)

1.0 ng/ml

1 vial

STANDARD C (0.5mL)

2.5 ng/ml

1 vial

STANDARD D (0.5mL)

5.0 ng/ml

1 vial

STANDARD E (0.5mL)

10 ng/ml

1 vial

STANDARD F (0.5mL)

25 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

GSK3a ELISA kit uses an anti-GSK3a antibody and an GSK3a-HRP conjugate in a competitive enzyme immunoassay method. GSK3a-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GSK3a antibody binding site between GSK3a from samples and GSK3a-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GSK3a. Since the number of sites is limited, as more sites are occupied by GSK3a from the sample, fewer sites are left to bind GSK3a-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GSK3a concentration in each sample is interpolated from this standard curve.


Quality Control on Mouse Glycogen Synthase Kinase 3 Alpha ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between GSK3a and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Glycogen Synthase Kinase 3 Alpha ELISA kit

Summary of the Assay Procedure for Mouse Glycogen Synthase Kinase 3 Alpha ELISA kit

Citations of Mouse Glycogen Synthase Kinase 3 Alpha ELISA kit

E03G0264 has been referenced in the below publications:

Effect of Different Factors of Moxibustion on P13K/AKT Signal Pathway and Cortical 6-amyioid Protein Precipitation in Brain Tissue of Alzheimer Mice.

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