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BlueGene Biotech Mouse Hepcidin ELISA kit (E03H0051)

E03H0051 Mouse Hepcidin ELISA kit

The Mouse Hepcidin ELISA kit can be used to identify samples from the mouse species. Hepcidin can also be called HAMP, HEPC, HFE2B, LEAP1, PLTR, hepcidin antimicrobial peptide, Hepc.

Products

Specifications of Mouse Hepcidin ELISA kit

Product Information

Cat. No.

E03H0051

Product Name

Mouse Hepcidin ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

25-500ng/mL

Sensitivity

1.0ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml1 vial

STANDARD B (0.5mL)

25 ng/ml

1 vial

STANDARD C (0.5mL)

50 ng/ml

1 vial

STANDARD D (0.5mL)

100 ng/ml

1 vial

STANDARD E (0.5mL)

250 ng/ml

1 vial

STANDARD F (0.5mL)

500 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Hepc ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-Hepc antibody and an Hepc-HRP conjugate. The assay sample and buffer are incubated together with Hepc-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Hepc concentration since Hepc from samples and Hepc-HRP conjugate compete for the anti-Hepc antibody binding site. Since the number of sites is limited, as more sites are occupied by Hepc from the sample, fewer sites are left to bind Hepc-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Hepc concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Hepcidin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Hepc and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Hepcidin ELISA kit

Summary of the Assay Procedure for Mouse Hepcidin ELISA kit

Citations of Mouse Hepcidin ELISA kit

E03H0051 has been referenced in the below publications:

Hydroxyurea Could Be a Good Clinically Relevant Iron Chelator.

n-Butanol soluble fraction of the water extract of Chinese toon fruit ameliorated focal brain ischemic insult in rats via inhibition of oxidative stress and inflammation.




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