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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Hormone Sensitive Lipase ELISA kit (E03H0281)

E03H0281 Mouse Hormone Sensitive Lipase ELISA kit

The Mouse Hormone Sensitive Lipase ELISA kit can be used to identify samples from the mouse species. Hormone Sensitive Lipase can also be called HSL, CEH, LIPE, AOMS4, FPLD6, HSL, LHS, lipase E, hormone sensitive type, REH, cholesteryl ester hydrolase.

Products

Specifications of Mouse Hormone Sensitive Lipase ELISA kit

Product Information

Cat. No.

E03H0281

Product Name

Mouse Hormone Sensitive Lipase ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

0.5-10ng/ml

Sensitivity

0.1ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml1 vial

STANDARD B (0.5mL)

0.5 ng/ml

1 vial

STANDARD C (0.5mL)

1.0 ng/ml

1 vial

STANDARD D (0.5mL)

2.5 ng/ml

1 vial

STANDARD E (0.5mL)

5.0ng/ml

1 vial

STANDARD F (0.5mL)

10 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

HSL ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-HSL antibody and a HSL-HRP conjugate. The assay sample and buffer are incubated together with HSL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSL concentration since HSL from samples and HSL-HRP conjugate compete for the anti-HSL antibody binding site. Since the number of sites is limited, as more sites are occupied by HSL from the sample, fewer sites are left to bind HSL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSL concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Hormone Sensitive Lipase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between HSL and analogues was observed.


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Summary of the Assay Procedure for Mouse Hormone Sensitive Lipase ELISA kit

Summary of the Assay Procedure for Mouse Hormone Sensitive Lipase ELISA kit

Citations of Mouse Hormone Sensitive Lipase ELISA kit

E03H0281 has been referenced in the below publications:

Effect of common edible oils on lipid metabolism and its mechanism in obese mice induced by a high fat diet.

Medium-Chain Triglyceride Activated Brown Adipose Tissue and Induced Reduction of Fat Mass in C57BL/6J Mice Fed High-fat Diet.


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