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  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Hyaluronic Acid ELISA kit (E03H0029)

E03H0029 Mouse Hyaluronic Acid ELISA kit

The Mouse Hyaluronic Acid ELISA kit can be used to identify samples from the mouse species. Hyaluronic Acid can also be called HA, hyaluronan, Hyaluronan, Hyaluronate.

Products

Specifications of Mouse Hyaluronic Acid ELISA kit

Product Information

Cat. No.

E03H0029

Product Name

Mouse Hyaluronic Acid ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

2.5-50ng/mL

Sensitivity

0.1ng/mL

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

HA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HA. Standards or samples are then added to the microtiter plate wells and HA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for HA are added to each well to sandwichthe HA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HA concentration in each sample is interpolated from this standard curve.


Quality Control on Mouse Hyaluronic Acid ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between HA selectin and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Hyaluronic Acid ELISA kit

Summary of the Assay Procedure for Mouse Hyaluronic Acid ELISA kit

Citations of Mouse Hyaluronic Acid ELISA kit

E03H0029 has been referenced in the below publications:

6% Hydroxyethyl starch (HES 130/0.4) diminishes glycocalyx degradation and decreases vascular permeability during systemic and pulmonary inflammation in mice.

The protective effect of 36 amino acid polypeptide fragment to CCl 4-induced liver fibrosis and its mechanism.

Isolation, Purification and Hepatoprotective Effect of Polysaccharides from Dicliptera chinensis.

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