E03I0057 Mouse Indoleamine 2,3 Dioxygenase ELISA kit
The Mouse Indoleamine 2,3 Dioxygenase ELISA kit can be used to identify samples from the mouse species. Indoleamine 2,3 Dioxygenase can also be called Indoleamine 2, 3-dioxygenase , 3-dioxygenase, IDO 1, INDO, Indole 2 3 dioxygenase, indoleamine 2 3 dioxygenase 1, indoleamine 2 3 dioxygenase, Indoleamine pyrrole 2 3 dioxygenase, Indoleamine-pyrrole 2.
Product Information | |
Cat. No. | E03I0057 |
Product Name | Mouse Indoleamine 2,3 Dioxygenase ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100ng/ml |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IDO ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IDO antibody and an IDO-HRP conjugate. The assay sample and buffer are incubated together with IDO-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IDO concentration since IDO from samples and IDO-HRP conjugate compete for the anti-IDO antibody binding site. Since the number of sites is limited, as more sites are occupied by IDO from the sample, fewer sites are left to bind IDO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IDO concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IDO and analogues was observed. | |
E03I0057 has been referenced in the below publications：
Phorbol ester activates human mesenchymal stem cells to inhibit B cells and ameliorate lupus symptoms in MRL.Faslpr mice.
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