Mouse Inducible Nitric Oxide Synthase ELISA kit (E03I0396)

BlueGene Biotech Mouse Inducible Nitric Oxide Synthase ELISA kit (E03I0396)

E03I0396 Mouse Inducible Nitric Oxide Synthase ELISA kit

The Mouse Inducible Nitric Oxide Synthase ELISA kit can be used to identify samples from the mouse species. Inducible Nitric Oxide Synthase can also be called INOS, Nitric Oxide Synthase, Inducible, HEP NOS, Hepatocyte NOS, HEPNOS, Inducible nitric oxide synthase, Inducible NO synthase, Inducible NOS, INOS, Inosl, MAC NOS, Macrophage NOS, iNOS.

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Specifications of Mouse Inducible Nitric Oxide Synthase ELISA kit

Product Information
Cat. No.	E03I0396
Product Name	Mouse Inducible Nitric Oxide Synthase ELISA kit
Species	Mouse
Product Size	48 Tests / 96 Tests
Concentration	2.5-50ng/ml
Sensitivity	0.1ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/ml	1 vial
STANDARD B (0.5mL)	2.5 ng/ml	1 vial
STANDARD C (0.5mL)	5.0 ng/ml	1 vial
STANDARD D (0.5mL)	10 ng/ml	1 vial
STANDARD E (0.5mL)	25 ng/ml	1 vial
STANDARD F (0.5mL)	50 ng/ml	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

iNOS ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-iNOS antibody and an iNOS-HRP conjugate. The assay sample and buffer are incubated together with iNOS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the iNOS concentration since iNOS from samples and iNOS-HRP conjugate compete for the anti-iNOS antibody binding site. Since the number of sites is limited, as more sites are occupied by iNOS from the sample, fewer sites are left to bind iNOS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The iNOS concentration in each sample is interpolated from this standard curve.

Quality Control on Mouse Inducible Nitric Oxide Synthase ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between iNOS and analogues was observed.

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Summary of the Assay Procedure for Mouse Inducible Nitric Oxide Synthase ELISA kit

Citations of Mouse Inducible Nitric Oxide Synthase ELISA kit

E03I0396 has been referenced in the below publications：

Environmental noise stress disturbs commensal microbiota homeostasis and induces oxi-inflammmation and AD-like neuropathology through epithelial barrier disruption in the EOAD mouse model.