Mouse Insulin Like Growth Factor Binding Protein 3 ELISA kit (E03I0074)

BlueGene Biotech Mouse Insulin Like Growth Factor Binding Protein 3 ELISA kit (E03I0074)

E03I0074 Mouse Insulin Like Growth Factor Binding Protein 3 ELISA kit

The Mouse Insulin Like Growth Factor Binding Protein 3 ELISA kit can be used to identify samples from the mouse species. Insulin Like Growth Factor Binding Protein 3 can also be called IGFBP-3, IGFBP3, BP-53, IBP3, insulin like growth factor binding protein 3．

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Specifications of Mouse Insulin Like Growth Factor Binding Protein 3 ELISA kit

Product Information
Cat. No.	E03I0074
Product Name	Mouse Insulin Like Growth Factor Binding Protein 3 ELISA kit
Species	Mouse
Product Size	48 Tests / 96 Tests
Concentration	0.5-10ng/ml
Sensitivity	0.1ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/ml	1 vial
STANDARD B (0.5mL)	0.5 ng/ml	1 vial
STANDARD C (0.5mL)	1.0 ng/ml	1 vial
STANDARD D (0.5mL)	2.5 ng/ml	1 vial
STANDARD E (0.5mL)	5.0ng/ml	1 vial
STANDARD F (0.5mL)	10 ng/ml	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

IGFBP3 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IGFBP3 antibody and a IGFBP3-HRP conjugate. The assay sample and buffer are incubated together with IGFBP3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IGFBP3 concentration since IGFBP3 from samples and IGFBP3-HRP conjugate compete for the anti-IGFBP3 antibody binding site. Since the number of sites is limited, as more sites are occupied by IGFBP3 from the sample, fewer sites are left to bind IGFBP3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGFBP3 concentration in each sample is interpolated from this standard curve.

Quality Control on Mouse Insulin Like Growth Factor Binding Protein 3 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between IGFBP3 and analogues was observed.

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Summary of the Assay Procedure for Mouse Insulin Like Growth Factor Binding Protein 3 ELISA kit

Citations of Mouse Insulin Like Growth Factor Binding Protein 3 ELISA kit

E03I0074 has been referenced in the below publications：

Tetrahydroxystilbene glucoside extends mouse life span via upregulating neural klotho and downregulating neural insulin or insulin-like growth factor 1.