Mouse Insulin Like Protein 5 ELISA kit (E03I0118) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Mouse Insulin Like Protein 5 ELISA kit (E03I0118)

E03I0118 Mouse Insulin Like Protein 5 ELISA kit

The Mouse Insulin Like Protein 5 ELISA kit can be used to identify samples from the mouse species. Insulin Like Protein 5 can also be called INSL5, PRO182, UNQ156, insulin like 5, Insulin-like peptide 5.

Products

Specifications of Mouse Insulin Like Protein 5 ELISA kit

Product Information
Cat. No.	E03I0118
Product Name	Mouse Insulin Like Protein 5 ELISA kit
Species	Mouse
Product Size	48 Tests / 96 Tests
Concentration	50-1000pg/ml
Sensitivity	1.0pg/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/ml	1 vial
STANDARD B (0.5mL)	50 pg/ml	1 vial
STANDARD C (0.5mL)	100 pg/ml	1 vial
STANDARD D (0.5mL)	250 pg/ml	1 vial
STANDARD E (0.5mL)	500 pg/ml	1 vial
STANDARD F (0.5mL)	1000 pg/ml	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

INSL5 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-INSL5 antibody and an INSL5-HRP conjugate. The assay sample and buffer are incubated together with INSL5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the INSL5 concentration since INSL5 from samples and INSL5-HRP conjugate compete for the anti-INSL5 antibody binding site. Since the number of sites is limited, as more sites are occupied by INSL5 from the sample, fewer sites are left to bind INSL5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The INSL5 concentration in each sample is interpolated from this standard curve.

Quality Control on Mouse Insulin Like Protein 5 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between INSL5 and analogues was observed.