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  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Lactoferrin ELISA kit (E03L0224)

E03L0224 Mouse Lactoferrin ELISA kit

The Mouse Lactoferrin ELISA kit can be used to identify samples from the mouse species. Lactoferrin can also be called  LF, HLF2, LF, GIG12, Lactotransferrin, Growth-inhibiting protein 12, Talalactoferrin, Lfcin-H, Kaliocin-1.

Products

Specifications of Mouse Lactoferrin ELISA kit

Product Information

Cat. No.

E03L0224

Product Name

Mouse Lactoferrin ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

250-5000pg/ml

Sensitivity

1.0pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

250 pg/mL

1 vial

STANDARD C (0.5mL)

500 pg/mL

1 vial

STANDARD D (0.5mL)

1000 pg/mL

1 vial

STANDARD E (0.5mL)

2500 pg/mL

1 vial

STANDARD F (0.5mL)

5000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

LF ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-LF antibody and an LF-HRP conjugate. The assay sample and buffer are incubated together with LF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LF concentration since LF from samples and LF-HRP conjugate compete for the anti-LF antibody binding site. Since the number of sites is limited, as more sites are occupied by LF from the sample, fewer sites are left to bind LF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LF concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Lactoferrin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between LF and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Lactoferrin ELISA kit

Summary of the Assay Procedure for Mouse Lactoferrin ELISA kit

Citations of Mouse Lactoferrin ELISA kit

E03L0224 has been referenced in the below publications:

Study on the regulation and mechanism that Mycobacterium Tuberculosis reacts at the iron metabolism of infected Mouse Alveolar macrophages.

Expression of TfR and Lf in Macrophages Infected with Different Virulence Mycobacterium Tuberculosis.


 


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