E03L0303 Mouse Lecithin Cholesterol Acyltransferase ELISA kit
The Mouse Lecithin Cholesterol Acyltransferase ELISA kit can be used to identify samples from the mouse species. Lecithin Cholesterol Acyltransferase can also be called phosphatidylcholine-sterol O-acyltransferase, Phospholipid-cholesterol acyltransferase, LCAT.
Product Information | |
Cat. No. | E03L0303 |
Product Name | Mouse Lecithin Cholesterol Acyltransferase ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000pg/ml |
Sensitivity | 1.0pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 250 pg/mL | 1 vial |
STANDARD C (0.5mL) | 500 pg/mL | 1 vial |
STANDARD D (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD E (0.5mL) | 2500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 5000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
LCAT ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-LCAT antibody and an LCAT-HRP conjugate. The assay sample and buffer are incubated together with LCAT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LCAT concentration since LCAT from samples and LCAT-HRP conjugate compete for the anti-LCAT antibody binding site. Since the number of sites is limited, as more sites are occupied by LCAT from the sample, fewer sites are left to bind LCAT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LCAT concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between LCAT and analogues was observed. | |
E03L0303 has been referenced in the below publications：
Effect of common edible oils on lipid metabolism and its mechanism in obese mice induced by a high fat diet.
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