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BlueGene Biotech Mouse Lecithin Cholesterol Acyltransferase ELISA kit (E03L0303)

E03L0303 Mouse Lecithin Cholesterol Acyltransferase ELISA kit

The Mouse Lecithin Cholesterol Acyltransferase ELISA kit can be used to identify samples from the mouse species. Lecithin Cholesterol Acyltransferase can also be called  phosphatidylcholine-sterol O-acyltransferase, Phospholipid-cholesterol acyltransferase, LCAT.

Products

Specifications of Mouse Lecithin Cholesterol Acyltransferase ELISA kit

Product Information

Cat. No.

E03L0303

Product Name

Mouse Lecithin Cholesterol Acyltransferase ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

250-5000pg/ml

Sensitivity

1.0pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

250 pg/mL

1 vial

STANDARD C (0.5mL)

500 pg/mL

1 vial

STANDARD D (0.5mL)

1000 pg/mL

1 vial

STANDARD E (0.5mL)

2500 pg/mL

1 vial

STANDARD F (0.5mL)

5000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

LCAT ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-LCAT antibody and an LCAT-HRP conjugate. The assay sample and buffer are incubated together with LCAT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LCAT concentration since LCAT from samples and LCAT-HRP conjugate compete for the anti-LCAT antibody binding site. Since the number of sites is limited, as more sites are occupied by LCAT from the sample, fewer sites are left to bind LCAT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LCAT concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Lecithin Cholesterol Acyltransferase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between LCAT and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Lecithin Cholesterol Acyltransferase ELISA kit

Summary of the Assay Procedure for Mouse Lecithin Cholesterol Acyltransferase ELISA kit

Citations of Mouse Lecithin Cholesterol Acyltransferase ELISA kit

E03L0303 has been referenced in the below publications:

Effect of common edible oils on lipid metabolism and its mechanism in obese mice induced by a high fat diet.


 


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