E03L0038 Mouse Leptin ELISA kit
The Mouse Leptin ELISA kit can be used to identify samples from the mouse species. Leptin can also be called LEP, Leptin Murine Obesity Homolog, Leptin Precursor Obesity Factor, OB, Obese Protein, Obesity, Obesity factor, Obesity homolog mouse, Obesity Murine Homolog Leptin, OBS, Leptin.
Product Information | |
Cat. No. | E03L0038 |
Product Name | Mouse Leptin ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50ng/ml |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/ml | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/ml | 1 vial |
STANDARD D (0.5mL) | 10 ng/ml | 1 vial |
STANDARD E (0.5mL) | 25 ng/ml | 1 vial |
STANDARD F (0.5mL) | 50 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Leptin ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-Leptin antibody and an Leptin-HRP conjugate. The assay sample and buffer are incubated together with Leptin-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Leptin concentration since Leptin from samples and Leptin-HRP conjugate compete for the anti-Leptin antibody binding site. Since the number of sites is limited, as more sites are occupied by Leptin from the sample, fewer sites are left to bind Leptin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Leptin concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Leptin and analogues was observed. | |
E03L0038 has been referenced in the below publications：
Influence of Electro-acupuncture on Serum Leptin and Adiponectin Level Expression in Obesity Mice with High Fat Diet.
Angl I-i nduced NOX4 Over expression Contributes to ROS Generation and Adipokine Secretion in 3T3-L1 Adipocyte CouId be Inhibited by Simvastatin: the Moleoular.
Effect of common edible oils on lipid metabolism and its mechanism in obese mice induced by a high fat diet.
Effect of Laminaria japonica Polysaccharide on Lepfin Level in Serum and Leptin Receptor Expression in Hypothalamus of Hyperlipemia Mice.
Resveratrol intake during pregnancy and lactation re-programs adiposity and ameliorates leptin resistance in male progeny induced by maternal high-fat/high sucrose plus postnatal high-fat/high
sucrose diets via fat metabolism regulation.
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