E03L0037 Mouse Leukotriene B4 ELISA kit
The Mouse Leukotriene B4 ELISA kit can be used to identify samples from the mouse species. Leukotriene B4 can also be called LTB4, LT-B4.
Specifications of Mouse Leukotriene B4 ELISA kit
Product Information | |
Cat. No. | E03L0037 |
Product Name | Mouse Leukotriene B4 ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000pg/ml |
Sensitivity | 1.0pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/ml | 1 vial |
STANDARD B (0.5mL) | 50 pg/ml | 1 vial |
STANDARD C (0.5mL) | 100 pg/ml | 1 vial |
STANDARD D (0.5mL) | 250 pg/ml | 1 vial |
STANDARD E (0.5mL) | 500 pg/ml | 1 vial |
STANDARD F (0.5mL) | 1000 pg/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
LTB4 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-LTB4 antibody and an LTB4-HRP conjugate. The assay sample and buffer are incubated together with LTB4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LTB4 concentration since LTB4 from samples and LTB4-HRP conjugate compete for the anti-LTB4 antibody binding site. Since the number of sites is limited, as more sites are occupied by LTB4 from the sample, fewer sites are left to bind LTB4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LTB4 concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Leukotriene B4 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between LTB4 and analogues was observed. | |
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Summary of the Assay Procedure for Mouse Leukotriene B4 ELISA kit
Citations of Mouse Leukotriene B4 ELISA kit
E03L0037 has been referenced in the below publications：
Mechanistic study of epidermal growth factor receptor inhibitor-related pruritus.
