E03L0001 Mouse Lipoprotein Lipase ELISA kit
The Mouse Lipoprotein Lipase ELISA kit can be used to identify samples from the mouse species. Lipoprotein Lipase can also be called LPL, Lipoprotein lipase, LIPD, LPL protein, HDLCQ11, LPL protein.
Specifications of Mouse Lipoprotein Lipase ELISA kit
Product Information | |
Cat. No. | E03L0001 |
Product Name | Mouse Lipoprotein Lipase ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 10-250ng/ml |
Sensitivity | 1.0ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 10 ng/mL | 1 vial |
STANDARD C (0.5mL) | 25 ng/mL | 1 vial |
STANDARD D (0.5mL) | 50 ng/mL | 1 vial |
STANDARD E (0.5mL) | 100 ng/mL | 1 vial |
STANDARD F (0.5mL) | 250 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
LPL ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-LPL antibody and an LPL-HRP conjugate. The assay sample and buffer are incubated together with LPL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LPL concentration since LPL from samples and LPL-HRP conjugate compete for the anti-LPL antibody binding site. Since the number of sites is limited, as more sites are occupied by LPL from the sample, fewer sites are left to bind LPL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LPL concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Lipoprotein Lipase ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between LPL and analogues was observed. | |
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Summary of the Assay Procedure for Mouse Lipoprotein Lipase ELISA kit
Citations of Mouse Lipoprotein Lipase ELISA kit
E03L0001 has been referenced in the below publications：
Effect of common edible oils on lipid metabolism and its mechanism in obese mice induced by a high fat diet.
