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BlueGene Biotech Mouse Methylglyoxal ELISA kit (E03M0040)

E03M0040 Mouse Methylglyoxal ELISA kit

The Mouse Methylglyoxal ELISA kit can be used to identify samples from the mouse species. Methylglyoxal can also be called Pyruvaldehyde, MG.

Products

Specifications of Mouse Methylglyoxal ELISA kit

Product Information

Cat. No.

E03M0040

Product Name

Mouse Methylglyoxal ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

5.0-100ng/ml

Sensitivity

1.0ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml

1 vial

STANDARD B (0.5mL)

5.0 ng/ml

1 vial

STANDARD C (0.5mL)

10 ng/ml

1 vial

STANDARD D (0.5mL)

25 ng/ml

1 vial

STANDARD E (0.5mL)

50 ng/ml

1 vial

STANDARD F (0.5mL)

100 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

MG ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MG antibody and an MG-HRP conjugate. The assay sample and buffer are incubated together with MG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MG concentration since MG from samples and MG-HRP conjugate compete for the anti-MG antibody binding site. Since the number of sites is limited, as more sites are occupied by MG from the sample, fewer sites are left to bind MG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MG concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Methylglyoxal ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between MG and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Methylglyoxal ELISA kit

Summary of the Assay Procedure for Mouse Methylglyoxal ELISA kit

Citations of Mouse Methylglyoxal ELISA kit

E03M0040 has been referenced in the below publications:

Metformin prevents methylglyoxal-induced apoptosis by suppressing oxidative stress in vitro and in vivo.

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