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BlueGene Biotech Mouse N Terminal Pro Brain Natriuretic Peptide ELISA kit (E03N0008)

E03N0008 Mouse N Terminal Pro Brain Natriuretic Peptide ELISA kit

The Mouse N Terminal Pro Brain Natriuretic Peptide ELISA kit can be used to identify samples from the mouse species. N Terminal Pro Brain Natriuretic Peptide can also be called BNPT, NT proBNP.

Products

Specifications of Mouse N Terminal Pro Brain Natriuretic Peptide ELISA kit

Product Information

Cat. No.

E03N0008

Product Name

Mouse N Terminal Pro Brain Natriuretic Peptide ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

100-2500pg/ml

Sensitivity

1.0pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

100 pg/mL

1 vial

STANDARD C (0.5mL)

250 pg/mL

1 vial

STANDARD D (0.5mL)

500 pg/mL

1 vial

STANDARD E (0.5mL)

1000 pg/mL

1 vial

STANDARD F (0.5mL)

2500 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

NT proBNP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-NT proBNP antibody and an NT proBNP-HRP conjugate. The assay sample and buffer are incubated together with NT proBNP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NT proBNP concentration since NT proBNP from samples and NT proBNP-HRP conjugate compete for the anti-NT proBNP antibody binding site. Since the number of sites is limited, as more sites are occupied by NT proBNP from the sample, fewer sites are left to bind NT proBNP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NT proBNP concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse N Terminal Pro Brain Natriuretic Peptide ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between NT proBNP and analogues was observed.


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Summary of the Assay Procedure for Mouse N Terminal Pro Brain Natriuretic Peptide ELISA kit

Summary of the Assay Procedure for Mouse N Terminal Pro Brain Natriuretic Peptide ELISA kit

Citations of Mouse N Terminal Pro Brain Natriuretic Peptide ELISA kit

E03N0008 has been referenced in the below publications:

Functional thyrotropin receptor expression in the ventricle and the effects on ventricular BNP secretion.


 


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