Mouse Nephrin ELISA kit (E03N0079) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Mouse Nephrin ELISA kit (E03N0079)

E03N0079 Mouse Nephrin ELISA kit

The Mouse Nephrin ELISA kit can be used to identify samples from the mouse species. Nephrin can also be called MIS/AMH, MIS_HUMAN, Anti muellerian hormone, MIF, Muellerian inhibiting factor, Mullerian inhibiting substance, Anti-Muellerian hormone, Nephrin.

Products

Specifications of Mouse Nephrin ELISA kit

Product Information
Cat. No.	E03N0079
Product Name	Mouse Nephrin ELISA kit
Species	Mouse
Product Size	48 Tests / 96 Tests
Concentration	1.0-25ng/ml
Sensitivity	0.1ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/ml	1 vial
STANDARD B (0.5mL)	1.0 ng/ml	1 vial
STANDARD C (0.5mL)	2.5 ng/ml	1 vial
STANDARD D (0.5mL)	5.0 ng/ml	1 vial
STANDARD E (0.5mL)	10 ng/ml	1 vial
STANDARD F (0.5mL)	25 ng/ml	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

Nephrin ELISA kit uses an anti-Nephrin antibody and an Nephrin-HRP conjugate in a competitive enzyme immunoassay method. Nephrin-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Nephrin antibody binding site between Nephrin from samples and Nephrin-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Nephrin. Since the number of sites is limited, as more sites are occupied by Nephrin from the sample, fewer sites are left to bind Nephrin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Nephrin concentration in each sample is interpolated from this standard curve.

Quality Control on Mouse Nephrin ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between Nephrin and analogues was observed.