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BlueGene Biotech Mouse Nuclear Factor Erythroid 2 Related Factor 2 ELISA kit (E03N0037)

E03N0037 Mouse Nuclear Factor Erythroid 2 Related Factor 2 ELISA kit

The Mouse Nuclear Factor Erythroid 2 Related Factor 2 ELISA kit can be used to identify samples from the mouse species. Nuclear Factor Erythroid 2 Related Factor 2 can also be called Nuclear factor-E2 related factor2, Nrf-2, HEBP1, NF E2 related factor 2, NFE2 related factor 2, NFE2L2, Nrf2, Nuclear factor erythroid 2 related factor 2, Nuclear factor erythroid derived 2 like 2.

Products

Specifications of Mouse Nuclear Factor Erythroid 2 Related Factor 2 ELISA kit

Product Information

Cat. No.

E03N0037

Product Name

Mouse Nuclear Factor Erythroid 2 Related Factor 2 ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Nrf2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-Nrf2 antibody and an Nrf2-HRP conjugate. The assay sample and buffer are incubated together with Nrf2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Nrf2 concentration since Nrf2 from samples and Nrf2-HRP conjugate compete for the anti-Nrf2 antibody binding site. Since the number of sites is limited, as more sites are occupied by Nrf2 from the sample, fewer sites are left to bind Nrf2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Nrf2 concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Nuclear Factor Erythroid 2 Related Factor 2 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Nrf2 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Nuclear Factor Erythroid 2 Related Factor 2 ELISA kit

Summary of the Assay Procedure for Mouse Nuclear Factor Erythroid 2 Related Factor 2 ELISA kit

Citations of Mouse Nuclear Factor Erythroid 2 Related Factor 2 ELISA kit

E03N0037 has been referenced in the below publications:

Environmental noise stress disturbs commensal microbiota homeostasis and induces oxi-inflammmation and AD-like neuropathology through epithelial barrier disruption in the EOAD mouse model.

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