E03O0853 Mouse Osteoglycin ELISA kit
The Mouse Osteoglycin ELISA kit can be used to identify samples from the mouse species. Osteoglycin can also be called OGN, OIF, SLRR3A, Mimecan Proteoglycan, Osteoinductive Factor．
Specifications of Mouse Osteoglycin ELISA kit
Product Information | |
Cat. No. | E03O0853 |
Product Name | Mouse Osteoglycin ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10ng/ml |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/ml | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/ml | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/ml | 1 vial |
STANDARD E (0.5mL) | 5.0ng/ml | 1 vial |
STANDARD F (0.5mL) | 10 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
OGN ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-OGN antibody and a OGN-HRP conjugate. The assay sample and buffer are incubated together with OGN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OGN concentration since OGN from samples and OGN-HRP conjugate compete for the anti-OGN antibody binding site. Since the number of sites is limited, as more sites are occupied by OGN from the sample, fewer sites are left to bind OGN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OGN concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Osteoglycin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between OGN and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Osteoglycin ELISA kit
Citations of Mouse Osteoglycin ELISA kit
E03O0853 has been referenced in the below publications：
Osteoglycin silencing exerts inhibitory effects on myocardial fibrosis and epithelial/endothelial-mesenchymal transformation in a mouse model of myocarditis.
