E03O0012 Mouse Ovalbumin Specific Immunoglobulin E ELISA kit
The Mouse Ovalbumin Specific Immunoglobulin E ELISA kit can be used to identify samples from the mouse species. Ovalbumin Specific Immunoglobulin E can also be called OVA-slgE.
Specifications of Mouse Ovalbumin Specific Immunoglobulin E ELISA kit
Product Information | |
Cat. No. | E03O0012 |
Product Name | Mouse Ovalbumin Specific Immunoglobulin E ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25μg/ml |
Sensitivity | 0.1μg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/ml | 1 vial |
STANDARD B (0.5mL) | 1.0 ug/ml | 1 vial |
STANDARD C (0.5mL) | 2.5 ug/ml | 1 vial |
STANDARD D (0.5mL) | 5.0 ug/ml | 1 vial |
STANDARD E (0.5mL) | 10 ug/ml | 1 vial |
STANDARD F (0.5mL) | 25 ug/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
OVA slgE ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-OVA slgE antibody and an OVA slgE-HRP conjugate. The assay sample and buffer are incubated together with OVA slgE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OVA slgE concentration since OVA slgE from samples and OVA slgE-HRP conjugate compete for the anti-OVA slgE antibody binding site. Since the number of sites is limited, as more sites are occupied by OVA slgE from the sample, fewer sites are left to bind OVA slgE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OVA slgE concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Ovalbumin Specific Immunoglobulin E ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between OVA slgE and analogues was observed. | |
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Summary of the Assay Procedure for Mouse Ovalbumin Specific Immunoglobulin E ELISA kit
Citations of Mouse Ovalbumin Specific Immunoglobulin E ELISA kit
E03O0012 has been referenced in the below publications：
Differential effects of formaldehyde exposure on airway inflammation and bronchial hyperresponsiveness in BALB/c and C57BL/6 mice.
Anti-asthmatic effect of Xuebijing and potential role of heme oxygenase-1 in an ovalbumin-induced murine allergic rhinitis model.
Period2 gene regulates diurnal changes of nasal symptoms in an allergic rhinitis mouse model.
Ameliorative effect of selective NLRP3 inflammasome inhibitor MCC950 in an ovalbumin-induced allergic rhinitis murine model.
Ca2+-activated K+ channel-3.1 blocker TRAM-34 alleviates murine allergic rhinitis.
