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BlueGene Biotech Mouse Ovalbumin Specific Immunoglobulin E ELISA kit (E03O0012)

E03O0012 Mouse Ovalbumin Specific Immunoglobulin E ELISA kit

The Mouse Ovalbumin Specific Immunoglobulin E ELISA kit can be used to identify samples from the mouse species. Ovalbumin Specific Immunoglobulin E can also be called OVA-slgE.

Products

Specifications of Mouse Ovalbumin Specific Immunoglobulin E ELISA kit

Product Information

Cat. No.

E03O0012

Product Name

Mouse Ovalbumin Specific Immunoglobulin E ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

1.0-25μg/ml

Sensitivity

0.1μg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ug/ml1 vial

STANDARD B (0.5mL)

1.0 ug/ml

1 vial

STANDARD C (0.5mL)

2.5 ug/ml

1 vial

STANDARD D (0.5mL)

5.0 ug/ml

1 vial

STANDARD E (0.5mL)

10 ug/ml

1 vial

STANDARD F (0.5mL)

25 ug/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

OVA slgE ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-OVA slgE antibody and an OVA slgE-HRP conjugate. The assay sample and buffer are incubated together with OVA slgE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OVA slgE concentration since OVA slgE from samples and OVA slgE-HRP conjugate compete for the anti-OVA slgE antibody binding site. Since the number of sites is limited, as more sites are occupied by OVA slgE from the sample, fewer sites are left to bind OVA slgE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OVA slgE concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Ovalbumin Specific Immunoglobulin E ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between OVA slgE and analogues was observed.


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Summary of the Assay Procedure for Mouse Ovalbumin Specific Immunoglobulin E ELISA kit

Summary of the Assay Procedure for Mouse Ovalbumin Specific Immunoglobulin E ELISA kit

Citations of Mouse Ovalbumin Specific Immunoglobulin E ELISA kit

E03O0012 has been referenced in the below publications:

Differential effects of formaldehyde exposure on airway inflammation and bronchial hyperresponsiveness in BALB/c and C57BL/6 mice.

Anti-asthmatic effect of Xuebijing and potential role of heme oxygenase-1 in an ovalbumin-induced murine allergic rhinitis model.

Period2 gene regulates diurnal changes of nasal symptoms in an allergic rhinitis mouse model.

Ameliorative effect of selective NLRP3 inflammasome inhibitor MCC950 in an ovalbumin-induced allergic rhinitis murine model.

Ca2+-activated K+ channel-3.1 blocker TRAM-34 alleviates murine allergic rhinitis.

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