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  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Oxidized Lowdensity Lipoprotein ELISA kit (E03O0341)

E03O0341 Mouse Oxidized Lowdensity Lipoprotein ELISA kit

The Mouse Oxidized Lowdensity Lipoprotein ELISA kit can be used to identify samples from the mouse species. Oxidized Lowdensity Lipoprotein can also be called  OL, OxLDL.

Products

Specifications of Mouse Oxidized Lowdensity Lipoprotein ELISA kit

Product Information

Cat. No.

E03O0341

Product Name

Mouse Oxidized Lowdensity Lipoprotein ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

5-100ug/ml

Sensitivity

1.0ug/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ug/mL

1 vial

STANDARD B (0.5mL)

5.0 ug/mL

1 vial

STANDARD C (0.5mL)

10 ug/mL

1 vial

STANDARD D (0.5mL)

25 ug/mL

1 vial

STANDARD E (0.5mL)

50 ug/mL

1 vial

STANDARD F (0.5mL)

100 ug/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

OxLDL ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-OxLDL antibody and an OxLDL-HRP conjugate. The assay sample and buffer are incubated together with OxLDL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OxLDL concentration since OxLDL from samples and OxLDL-HRP conjugate compete for the anti-OxLDL antibody binding site. Since the number of sites is limited, as more sites are occupied by OxLDL from the sample, fewer sites are left to bind OxLDL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OxLDL concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Oxidized Lowdensity Lipoprotein ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between OxLDL and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Oxidized Lowdensity Lipoprotein ELISA kit

Summary of the Assay Procedure for Mouse Oxidized Lowdensity Lipoprotein ELISA kit

Citations of Mouse Oxidized Lowdensity Lipoprotein ELISA kit

E03O0341 has been referenced in the below publications:

Simvastatin ameliorates renal lipidosis through the suppression of renal CXCL16 expression in mice with adriamycin-induced nephropathy.

Role of CXCL16 in mice with adriamycin induced nephropathy and the protective effects of simvastatin.

Molecular hydrogen stabilizes atherosclerotic plaque in low-density lipoprotein receptor-knockout mice.


 


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