E03P0572 Mouse Parathormone Intact ELISA kit
The Mouse Parathormone Intact ELISA kit can be used to identify samples from the mouse species. Parathormone Intact can also be called PTH, PTH1, parathyroid hormone, Parathyroid hormone, FIH1T.
Specifications of Mouse Parathormone Intact ELISA kit
Product Information | |
Cat. No. | E03P0572 |
Product Name | Mouse Parathormone Intact ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000pg/ml |
Sensitivity | 1.0pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/ml | 1 vial |
STANDARD B (0.5mL) | 50 pg/ml | 1 vial |
STANDARD C (0.5mL) | 100 pg/ml | 1 vial |
STANDARD D (0.5mL) | 250 pg/ml | 1 vial |
STANDARD E (0.5mL) | 500 pg/ml | 1 vial |
STANDARD F (0.5mL) | 1000 pg/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PTH ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PTH antibody and an PTH-HRP conjugate. The assay sample and buffer are incubated together with PTH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTH concentration since PTH from samples and PTH-HRP conjugate compete for the anti-PTH antibody binding site. Since the number of sites is limited, as more sites are occupied by PTH from the sample, fewer sites are left to bind PTH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTH concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Parathormone Intact ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PTH and analogues was observed. | |
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Summary of the Assay Procedure for Mouse Parathormone Intact ELISA kit
Citations of Mouse Parathormone Intact ELISA kit
E03P0572 has been referenced in the below publications：
Triglyceride with medium-chain fatty acids increases the activity and expression of hormone-sensitive lipase in white adipose tissue of C57BL/6J mice.
