E03P0574 Mouse Parathyroid Hormone Related Protein ELISA kit
The Mouse Parathyroid Hormone Related Protein ELISA kit can be used to identify samples from the mouse species. Parathyroid Hormone Related Protein can also be called PTHR, Parathyroid Hormone Receptor, PTHrP.
Product Information | |
Cat. No. | E03P0574 |
Product Name | Mouse Parathyroid Hormone Related Protein ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000pg/ml |
Sensitivity | 1.0pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/ml | 1 vial |
STANDARD B (0.5mL) | 50 pg/ml | 1 vial |
STANDARD C (0.5mL) | 100 pg/ml | 1 vial |
STANDARD D (0.5mL) | 250 pg/ml | 1 vial |
STANDARD E (0.5mL) | 500 pg/ml | 1 vial |
STANDARD F (0.5mL) | 1000 pg/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PTHrP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PTHrP antibody and an PTHrP-HRP conjugate. The assay sample and buffer are incubated together with PTHrP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTHrP concentration since PTHrP from samples and PTHrP-HRP conjugate compete for the anti-PTHrP antibody binding site. Since the number of sites is limited, as more sites are occupied by PTHrP from the sample, fewer sites are left to bind PTHrP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTHrP concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PTHrP and analogues was observed. | |
E03P0574 has been referenced in the below publications：
Triglyceride with medium-chain fatty acids increases the activity and expression of hormone-sensitive lipase in white adipose tissue of C57BL/6J mice.
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